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KMID : 0545120000100030327
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 3 p.327 ~ p.332
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Cryopreservation of Suspension Cultures of Camptotheca acuminata
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Kim Sung-Hye
Byun Sang-Yo
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Abstract
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Suspended cells of Camptotheca acuminata were observed to lose their ability to synthesize camptothecin and its derivatives as a result of repeated cultures. Accordingly, the maintenance of high-yield cells by cryopreservation was studied to overcome this stability problem, and various factors involved were optimized. Pregrowing the cells in 8% myoinositol for 4 days was found to be the most effective in improving survival. The highest survival was obtained when the pregrown cells were cryoprotected with a mixture of 10% DMSO, 0.6M mannitol, and 10% glycerol. When the cryopreserved cells were maintained in a freezer at -70℃, 94% survival was obtained after 4 months. The survivals after 5 and 8 months of storage decreased to 52% and 45%, respectively. No loss of biosynthetic capability of camptothecin was observed after short to medium term cryopreservation of C. acuminata.
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